RPH3A anticorps (pSer234)

N° du produit ABIN361489

Cet anticorps Lapin Polyclonal détecte spécifiquement RPH3A dans WB et IHC. Il présente une réactivité envers Rat et a été mentionné dans 1 publication.

Aperçu rapide pour RPH3A anticorps (pSer234) (ABIN361489)

Antigène: RPH3A (Rabphilin 3A (RPH3A))

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RPH3A est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-RPH3A anticorps

Épitope: pSer234

Specificité: Specific for the ~82k rabphilin protein phosphorylated at Ser234. The immunolabeling of the rabphilin band was completely blocked by the phosphopeptide used as antigen while the dephosphopeptide had no effect on the immunolabeling

Réactivité croisée: Rat (Rattus)

Homologie: mouse

Purification: Antigen Affinity Purified from Pooled Serum

Immunogène: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser234 conjugated to KLH

Information d'application

Indications d'application: Recommended Dilution: WB: 1:1000 IHC: 1:400 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Stock: -20 °C

Références pour anticorps anti-RPH3A (ABIN361489)

Stephens, Powell, Taraska, Harris: "Imaging the rapid yet transient accumulation of regulatory lipids, lipid kinases, and protein kinases during membrane fusion, at sites of exocytosis of MMP-9 in MCF-7 cells." dans: Lipids in health and disease, Vol. 19, Issue 1, pp. 195, (2020) (PubMed).

Détails sur RPH3A

Antigène: RPH3A (Rabphilin 3A (RPH3A))

Autre désignation: RPH3A

Sujet: Rabphilin-3A is a peripheral membrane protein that binds Ca2+ and phospholipids and is attached to synaptic vesicle membranes (Geppert et al., 1994,Li et al., 1994). The expression of rabphilin 3A is reduced in an animal model of Huntington’s disease(Smith et al., 2005). Rabphilin can be phosphorylated at Ser234 by both CAM kinase II and PKA and this phosphorylation has been suggested to regulate neuronal activity during development in a synapse-specific manner (Fykse et al., 1995,Foletti and Scheller, 2001). Anti-Phospho-Ser234 Rabphilin 3A Western blot of rat brain lysate showing specific immunolabeling of the ~82k rabphilin 3A phosphorylated at Ser234.

Poids moléculaire: '82 kDa

ID gène: 171039

UniProt: P47709

Pathways: Synaptic Vesicle Exocytosis